genotyping pcr conditions Search Results


human oc cell lines es2  (ATCC)
97
ATCC human oc cell lines es2
RBM15 expression in OC cell lines and OC tissues. (A) Detection of RBM15 mRNA expression in immortal ovarian surface epithelial cells (IOSE-80) and epithelial ovarian cancer cells (OVCAR-3, SK-OV-3, <t>ES2</t> and A2780) by reverse transcription-quantitative PCR. One-way ANOVA followed by the Dunnett test was used. Data presented as mean ± SD (n=3). (B) Detection of RBM15 protein expression in ovarian cell lines by western blot analysis. Representative images are shown. (C) Semiquantitative analysis of blots in (B). One-way ANOVA followed by the Dunnett test was used. Data presented as mean ± SD (n-3). (D) Detection of RBM15 protein expression in non-tumorous ovarian tissue and primary ovarian cancer tissue by immunohistochemistry staining. Representative images are shown. Original magnification ×100; Scale bar, 200 μ m. Partial magnification ×400; Scale bar, 50 μ m; (E) Comparison of RBM15 protein expression between non-tumorous ovarian tissue (n=9) and primary ovarian cancer tissue (n=18) using the Mann-Whitney test. Data presented as mean ± SD. ns, not significant (P>0.05). RBM15, RNA binding motif protein 15; OC, ovarian cancer; SI, staining index.
Human Oc Cell Lines Es2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genotyping+pcr+conditions/ES-2/pmc10552772-32-0-22
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standard pcr conditions  (Qiagen)
99
Qiagen standard pcr conditions
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Standard Pcr Conditions, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper dbgap phs001875 v1 p1 experimental models  (DSMZ)
94
DSMZ paper dbgap phs001875 v1 p1 experimental models
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Paper Dbgap Phs001875 V1 P1 Experimental Models, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gotaq green master mix  (Promega)
90
Promega gotaq green master mix
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Gotaq Green Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper dbgap phs001707 v3 p1 experimental models  (ATCC)
97
ATCC paper dbgap phs001707 v3 p1 experimental models
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Paper Dbgap Phs001707 V3 P1 Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genotyping+pcr+conditions/P-1/pm37776850-258-43-53
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genotyping  (Transnetyx)
99
Transnetyx genotyping
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Genotyping, supplied by Transnetyx, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genotyping+pcr+conditions/Automated+Genotyping/pm33763075-295-15-19
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2018 dbgap phs001444 dlbcl  (ATCC)
96
ATCC 2018 dbgap phs001444 dlbcl
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
2018 Dbgap Phs001444 Dlbcl, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genotyping+pcr+conditions/SV40LT-SMC+Clone+HEP-SA/pm34380064-287-263-286
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standard pcr genotyping  (Jackson Laboratory)
86
Jackson Laboratory standard pcr genotyping
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Standard Pcr Genotyping, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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standard pcr genotyping - by Bioz Stars, 2026-09
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nebnext library quant kit for illumina  (New England Biolabs)
96
New England Biolabs nebnext library quant kit for illumina
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Nebnext Library Quant Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genotyping+pcr+conditions/NEBNext+Library+Quant+Kit+for+Illumina/custom%40e7630%4030529891
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nebnext library quant kit for illumina - by Bioz Stars, 2026-09
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17935-1  (agena bioscience)
99
agena bioscience 17935-1
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
17935 1, supplied by agena bioscience, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray system  (agena bioscience)
97
agena bioscience massarray system
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Massarray System, supplied by agena bioscience, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acanthamoeba castellanii neff american type culture collection strain sampling area locality nna pcr species genotype genebank  (ATCC)
96
ATCC acanthamoeba castellanii neff american type culture collection strain sampling area locality nna pcr species genotype genebank
Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron <t>41</t> <t>(genotyped</t> by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut <t>PCR</t> product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.
Acanthamoeba Castellanii Neff American Type Culture Collection Strain Sampling Area Locality Nna Pcr Species Genotype Genebank, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


RBM15 expression in OC cell lines and OC tissues. (A) Detection of RBM15 mRNA expression in immortal ovarian surface epithelial cells (IOSE-80) and epithelial ovarian cancer cells (OVCAR-3, SK-OV-3, ES2 and A2780) by reverse transcription-quantitative PCR. One-way ANOVA followed by the Dunnett test was used. Data presented as mean ± SD (n=3). (B) Detection of RBM15 protein expression in ovarian cell lines by western blot analysis. Representative images are shown. (C) Semiquantitative analysis of blots in (B). One-way ANOVA followed by the Dunnett test was used. Data presented as mean ± SD (n-3). (D) Detection of RBM15 protein expression in non-tumorous ovarian tissue and primary ovarian cancer tissue by immunohistochemistry staining. Representative images are shown. Original magnification ×100; Scale bar, 200 μ m. Partial magnification ×400; Scale bar, 50 μ m; (E) Comparison of RBM15 protein expression between non-tumorous ovarian tissue (n=9) and primary ovarian cancer tissue (n=18) using the Mann-Whitney test. Data presented as mean ± SD. ns, not significant (P>0.05). RBM15, RNA binding motif protein 15; OC, ovarian cancer; SI, staining index.

Journal: International Journal of Oncology

Article Title: RBM15-mediating MDR1 mRNA m 6 A methylation regulated by the TGF-β signaling pathway in paclitaxel-resistant ovarian cancer

doi: 10.3892/ijo.2023.5560

Figure Lengend Snippet: RBM15 expression in OC cell lines and OC tissues. (A) Detection of RBM15 mRNA expression in immortal ovarian surface epithelial cells (IOSE-80) and epithelial ovarian cancer cells (OVCAR-3, SK-OV-3, ES2 and A2780) by reverse transcription-quantitative PCR. One-way ANOVA followed by the Dunnett test was used. Data presented as mean ± SD (n=3). (B) Detection of RBM15 protein expression in ovarian cell lines by western blot analysis. Representative images are shown. (C) Semiquantitative analysis of blots in (B). One-way ANOVA followed by the Dunnett test was used. Data presented as mean ± SD (n-3). (D) Detection of RBM15 protein expression in non-tumorous ovarian tissue and primary ovarian cancer tissue by immunohistochemistry staining. Representative images are shown. Original magnification ×100; Scale bar, 200 μ m. Partial magnification ×400; Scale bar, 50 μ m; (E) Comparison of RBM15 protein expression between non-tumorous ovarian tissue (n=9) and primary ovarian cancer tissue (n=18) using the Mann-Whitney test. Data presented as mean ± SD. ns, not significant (P>0.05). RBM15, RNA binding motif protein 15; OC, ovarian cancer; SI, staining index.

Article Snippet: Human OC cell lines ES2 [originating from ovarian clear cell carcinoma but the genetic profile closely related to serous carcinoma ( ); ATCC], A2780 (originating from ovarian endometrioid adenocarcinoma) and its PTX-resistant counterpart A2780-PTX (Nanjing KeyGen Biotech Co., Ltd.) were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Staining, Comparison, MANN-WHITNEY, RNA Binding Assay

Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron 41 (genotyped by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut PCR product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Immortalization of human normal and NF1 neurofibroma Schwann cells.

doi: 10.1038/labinvest.2016.88

Figure Lengend Snippet: Figure 1 Molecular analysis of two immortalized plexiform SC lines. (a) Loss of heterozygosity analysis of pNF95.11b, using SNP marker rs964288 in the NF1 gene intron 41 (genotyped by PacI digestion). Lane 1 contains a molecular weight ladder. Lane 2 shows an uncut PCR product for reference. Lane 3 shows PacI digest of the PCR product from the patient’s leukocyte (germline) DNA, showing heterozygous genotype. Lane 4 contains the PacI digest from the primary tumor PCR product, showing slight loss of heterozygosity, consistent with tissue admixture. Lane 5 shows the genotype of the primary tumor SC culture (p8) showing some enrichment for the two-hit SC. Lane 6 shows that immortalized culture “C/T” (p43) has no evidence of the wild-type allele despite overloading, as also seen in lane 7 for the “C” line (p41). (b) A sequencing chromatogram shows approximately equal quantities of the “C” germline allele and the somatic mutant “T” allele in SC line ipNF95.6 (p42) at cDNA position 6709 (NM_000267.3) (shown as “N” on chromatogram) encoding R2237X nonsense mutation.

Article Snippet: Microsatellite Instability D5S346, D17S250, and BAT26, three established NCI microsatellite instability markers,38 were genotyped using standard PCR conditions (HotStar Taq polymerase, Qiagen, Valencia, CA, USA) followed by 15% native polyacrylamide gel electrophoresis to screen for de novo alleles in the cells' DNA compared with that of primary cultures or germline DNA.

Techniques: Marker, Molecular Weight, Sequencing, Mutagenesis